Application of Gas Liquid Chromatography in the Fusariotoxin Analysis (Trychothecene, Zearalenone)

Gas-liquid chromatography (GLC) 1s commonly used for the identification and quantitative determination of a number of mycotoxins in foodstuffs and fodder. In particular, the techniques involving GLC have been developed for the analysis of trichothecen mycotoxins (T-2 and HT-2 toxins, diacetoxyscirpenol, deoxynivalenol, nivalenol, etc.), zearalenone, patulin and some others. The GLO technique is characterized by high partition effect, adequate reproducibility of results and high sensitivity. The subject of the present seminar is to discuss praclical aspects of this technique for the analysis of fusariotoxins using the examples of T-2 toxin, zearalenone and a mixture of trichothecens, isolated from contaminated grain.