Multidetection of Mycotoxins in Grain Extracts and Fungi Cultures

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dc.contributor.authorFood and Agriculture Organization of the United Nations
dc.contributor.authorUnited Nations Environment Programme
dc.contributor.organizationEconomy Division
dc.contributor.otherLvova, L. S.
dc.contributor.otherDoronina, O.D.
dc.date.accessioned2019-04-19T20:46:02Z
dc.date.available2019-04-19T20:46:02Z
dc.date.issued1984
dc.descriptionThis document presents an experimental methodology for the simultaneous detection and semi-quantitative screening of multiple mycotoxins in grain extracts and fungi cultures. It details the underlying causes for the co-occurrence of various mycotoxins and provides step-by-step protocols encompassing extraction, degreasing, purification (using techniques such as dialysis, gel-filtration with Sephadex LH-20, and silica gel columns), and thin-layer chromatography on “Silufol” plates. The text specifies solvent systems and reagent preparations for optimal chromatographic separation and identification, utilizing both fluorescence under UV light and colorimetric reactions unique to each toxin. The detection thresholds for numerous mycotoxins—including aflatoxins, ochratoxin A, citrinin, zearalenone, and others—are cataloged, enabling semi-quantitative estimations by comparison with standards.en
dc.formatText
dc.identifier.urihttps://wedocs.unep.org/handle/20.500.11822/28026
dc.languageEnglish
dc.page.number14 p.
dc.rights.accessLevelPublic
dc.subjectfungi
dc.subjecttoxic substance
dc.subjectgrain
dc.subjectphosphoric acid
dc.titleMultidetection of Mycotoxins in Grain Extracts and Fungi Culturesen
dc.typeCommunication or other resource

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