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T1 Multidetection of Mycotoxins in Grain Extracts and Fungi Cultures
A1 Food and Agriculture Organization of the United Nations, 
A1 United Nations Environment Programme, 
A2 Lvova, L. S.
A2 Doronina, O.D.
K1 fungi
K1 toxic substance
K1 grain
K1 phosphoric acid
YR 1984
FD 1984
LK https://wedocs.unep.org/handle/20.500.11822/28026
UL https://wedocs.unep.org/handle/20.500.11822/28026
NO This document presents an experimental methodology for the simultaneous detection and semi-quantitative screening of multiple mycotoxins in grain extracts and fungi cultures. It details the underlying causes for the co-occurrence of various mycotoxins and provides step-by-step protocols encompassing extraction, degreasing, purification (using techniques such as dialysis, gel-filtration with Sephadex LH-20, and silica gel columns), and thin-layer chromatography on “Silufol” plates. The text specifies solvent systems and reagent preparations for optimal chromatographic separation and identification, utilizing both fluorescence under UV light and colorimetric reactions unique to each toxin. The detection thresholds for numerous mycotoxins—including aflatoxins, ochratoxin A, citrinin, zearalenone, and others—are cataloged, enabling semi-quantitative estimations by comparison with standards.
DS UN Environment Document Repository
RD Sep 23, 2026